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SOP:Validation of U.V Light Sterilization

This SOP outlines the validation procedure for UV light sterilization in the microbiology lab. It details responsibilities, procedures for inoculation, plate distribution, UV exposure, incubation, and acceptance criteria. Validation is performed every 100 burning hours to ensure effective sterilization, maintaining compliance with quality and safety standards.

BIOLOGICAL

1 min read

1.0 OBJECTIVE

1.1 To lay down the procedure for validation of U.V light sterilization.

2.0 SCOPE

2.1 This SOP shall be applicable for validation of U.V light sterilization in the Microbiology Lab.

3.0 RESPONSIBILITY

3.1 The Microbiologist and above shall be responsible for the preparation and execution of this SOP.
3.2 The QC & QA Head shall be responsible for the checking and approval of this SOP.
3.3 The HOD shall be responsible for the authorization of this SOP.

4.0 ACCOUNTABILITY

4.1 All concerned personnel shall ensure compliance with this SOP.

5.0 PROCEDURE

5.1 Preparation of Agar Plates

5.1.1 Prepare Soybean Casein Digest Agar plates (SCDA)

5.2 Inoculation

5.2.1 Take four Soybean Casein Digest Agar plates.
5.2.2 Inoculate each plate with 10–20 cfu of Bacillus subtilis, Escherichia coli, Staphylococcus aureus, etc.

5.3 Plate Distribution

5.3.1 Mark two plates as "control" and incubate them at 35°–37°C for 48 hours without exposure to UV light.
5.3.2 Mark the other two plates for UV exposure and proceed as follows:

Place them under the UV light to be validated.

5.4 UV Light Exposure

5.4.1 Turn on the UV light and expose the marked plates for one hour.

5.5 Incubation

5.5.1 After exposure, incubate the plates at 35°–37°C for 72 hours.

5.6 Observations

5.6.1 Observe the plates for the number of colonies.

5.7 Acceptance Criteria

5.7.1 The number of colonies observed in the UV-exposed plates should be 10 times less than the colonies observed in the control plates.

6.0 FREQUENCY

6.1 Perform the validation after every 100 burning hours of the UV light.

7.0 ANNEXURE

7.1 Format for recording the result

8.0 ABBREVIATIONS

SOP: Standard Operating Procedure
QC: Quality Control
QA: Quality Assurance
QCD: Quality Control Department
No.: Number
N.A: Not Applicable
I.P.A: Iso Propyl Alcohol
Lab.: Laboratory